anti jnk  (Proteintech)

Journal: Frontiers in Immunology

Article Title: Platelet-rich plasma-derived microRNA let-7a-5p alleviates knee osteoarthritis by regulating macrophage polarization and improving inflammatory microenvironment

doi: 10.3389/fimmu.2026.1756467

Figure Lengend Snippet: Validation of the targeting relationship between let-7a-5p and MAPK8. (A) Schematic representation of the predicted complementary binding site between let-7a-5p and the 3′-UTR of MAPK8. (B) Relative mRNA expression level of MAPK8 in macrophages overexpressing let-7a-5p detected by RT-qPCR. (C) WB analysis of MAPK8 protein expression in macrophages overexpressing let-7a-5p. (D) Inhibitory effect of let-7a-5p on MAPK8 expression was assessed via a dual luciferase reporter assay. ( **P < 0.01, ***P < 0.001).

Article Snippet: Subsequently, the tissue sections and cells were incubated overnight at 4°C with primary antibodies against iNOS (Proteintech 22226-1-AP), CD206 (Proteintech 18704-1-AP) and MAPK8 (Proteintech 66210-1-Ig).

Techniques: Biomarker Discovery, Binding Assay, Expressing, Quantitative RT-PCR, Luciferase, Reporter Assay

Journal: Frontiers in Immunology

Article Title: Platelet-rich plasma-derived microRNA let-7a-5p alleviates knee osteoarthritis by regulating macrophage polarization and improving inflammatory microenvironment

doi: 10.3389/fimmu.2026.1756467

Figure Lengend Snippet: Effect of PRP on expression of let-7a-5p and MAPK8. (A) Relative mRNA expression levels of let-7a-5p in knee joint sections of each group detected by RT-qPCR. (B) iNOS and CD206 co-immunolabeld with MAPK8 and counter-stained with DAPI in synovial tissues (Scale bar: 50 μm). (C, D) Quantification of iNOS and CD206 expression co-localized with MAPK8. ( **P < 0.01).

Article Snippet: Subsequently, the tissue sections and cells were incubated overnight at 4°C with primary antibodies against iNOS (Proteintech 22226-1-AP), CD206 (Proteintech 18704-1-AP) and MAPK8 (Proteintech 66210-1-Ig).

Techniques: Expressing, Quantitative RT-PCR, Staining

Journal: Frontiers in Immunology

Article Title: Platelet-rich plasma-derived microRNA let-7a-5p alleviates knee osteoarthritis by regulating macrophage polarization and improving inflammatory microenvironment

doi: 10.3389/fimmu.2026.1756467

Figure Lengend Snippet: The let-7a-5p/MAPK8 axis regulates macrophage polarization and inflammatory cytokine release in vitro . (A) IF staining showing expression of iNOS and CD206 in macrophages (Scale bar: 50 μm). (B) Quantification of iNOS-positive cell rate in transfected macrophages. (C) Quantification of CD206-positive cell rate in transfected macrophages. (D-G) Relative mRNA expression levels of pro-inflammatory cytokine (IL-1β and TNF-α) and anti-inflammatory cytokine (IL-4 and IL-10) in transfected macrophages detected by RT-qPCR. ( *P < 0.05, **P < 0.01, ns no significance).

Article Snippet: Subsequently, the tissue sections and cells were incubated overnight at 4°C with primary antibodies against iNOS (Proteintech 22226-1-AP), CD206 (Proteintech 18704-1-AP) and MAPK8 (Proteintech 66210-1-Ig).

Techniques: In Vitro, Staining, Expressing, Transfection, Quantitative RT-PCR

Journal: Scientific reports

Article Title: Mitigating doxorubicin-induced hepatotoxicity in male rats: The role of aerobic interval training and curcumin supplementation in reducing oxidative stress, endoplasmic reticulum stress and apoptosis.

doi: 10.1038/s41598-025-91133-6

Figure Lengend Snippet: Fig. 4. Protein expression of endoplasmic reticulum stress markers in the hepatic tissue (A) representative western blot analysis of ER stress markers in the hepatic tissue; (B) p-IRE1ɑ/IRE1ɑ ratio; (C) ATF6/GAPDH; (D) p-JNK/JNK ratio; (E) p-PERK/PERK ratio; (F) GRP78/GAPDH; (G) ATF4/GAPDH; (H) p-eIF2ɑ/eIF2ɑ ratio; (I) CHOP/GAPDH. Data are presented as mean ± SD (n = 4/group). * P < 0.05 vs. the CON group; # P < 0.05 vs. the Dox group; $ P < 0.05 vs. the Dox-C group. Abbreviations: ATF4, activating transcription factor 4; ATF6, activating transcription factor 6; CHOP; C/EBP homologous protein; eIF2ɑ, eukaryotic initiation factor 2ɑ; GRP78, the 78-kDa glucose-regulated protein; IRE1ɑ, inositol-requiring transmembrane kinase/ endoribonuclease 1α; JNK, c-Jun N-terminal Kinase; PERK, protein Kinase RNA-Like ER Kinase; CON, control group; Dox, doxorubicin group; Dox-C, doxorubicin + curcumin group; Dox-A, doxorubicin + aerobic interval training group; Dox-AC, doxorubicin + curcumin + aerobic interval training group.

Article Snippet: No. 8170721000), skim milk (Sigma-Aldrich), and Tris-buffered saline, glycerol, and distilled water), the PVDF membranes were blocked, followed by incubating with primary antibodies, including GRP78 (GeneTex, Cat. No. GTX113340), ATF6 (GeneTex, Cat. No. GTX15457), PERK (Cell Signaling Technology, Cat. No. #3192), phospho-PERK (Cell Signaling Technology, Cat. No. #12,185), ATF4 (GeneTex, Cat. No. GTX101943), eIF2ɑ (GeneTex, Cat. No. GTX101241), phosphoeIF2ɑ (GeneTex, Cat. No. GTX130006), CHOP (GeneTex, Cat. No. GTX112827), IRE1ɑ (GeneTex, Cat. No. GTX30005), phospho-IRE1ɑ (GeneTex, Cat. No. GTX132808), JNK (Biorbyt, Cat. No. orb129654), phosphoJNK (GeneTex, Cat. No. GTX52328), Bax (GeneTex, Cat. No. GTX109683), Bcl-2 (Biorbyt, Cat. No. orb10173), caspase-3 (Sigma-Aldrich, Cat. No. AB3623), caspase-12 (GeneTex, Cat. No. GTX37762), GAPDH (GeneTex, Cat. No. GTX100118) at a dilution of 1:1000 overnight at 4 °C.

Techniques: Expressing, Western Blot, Control